Antimicrobial discs are a crucial component in the assessment of bacterial resistance and the effectiveness of antimicrobial agents. The AffiBIOTICS Antimicrobial Discs are designed for use in agar diffusion methods, such as the Kirby-Bauer test, to determine the susceptibility of microorganisms to specific antibiotics.
Technical Content:
- Composition and Manufacturing: AffiBIOTICS Antimicrobial Discs are composed of a standard, high-purity filter paper impregnated with precise concentrations of antimicrobial agents. Each disc is manufactured under stringent quality control to ensure uniformity in drug content and disc integrity. The discs are sterilized to prevent contamination and are packaged in a controlled environment to maintain their efficacy.
- Mechanism of Action: The discs function through the diffusion of antimicrobial agents into the agar medium. When placed on an inoculated agar plate, the antibiotic diffuses radially, creating a concentration gradient. The effectiveness of the antimicrobial agent is indicated by the size of the inhibition zone around the disc. This zone reflects the drug's ability to inhibit bacterial growth, with larger zones indicating higher susceptibility.
- Usage Protocol:
- Preparation: Inoculate an agar plate with a standardized bacterial suspension.
- Application: Place the antimicrobial discs on the surface of the agar plate.
- Incubation: Incubate the plates at the recommended temperature for a specified duration, typically 24 hours.
- Measurement: After incubation, measure the diameter of the inhibition zone around each disc using calipers or a zone reader. Compare the results with standardized reference tables to determine susceptibility.
- Applications:
AffiBIOTICS Antimicrobial Discs are utilized in clinical diagnostics, research laboratories, and pharmaceutical development to:
- Determine bacterial resistance patterns.
- Guide therapeutic decisions by identifying effective antibiotics.
- Monitor the emergence of antibiotic-resistant strains.
- Quality Assurance: Each batch of AffiBIOTICS Antimicrobial Discs undergoes rigorous testing for consistency and performance. This includes calibration against reference strains, validation of disc potency, and sterility testing to ensure reliable results.
AffiBIOTICS Antimicrobial Discs are essential tools in antimicrobial susceptibility testing, offering precision and reliability in assessing the effectiveness of antibiotics against pathogenic microorganisms.
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