Cefotetan Susceptibility Testing 

Cefotetan is a second-generation cephalosporin with extended activity against Gram-negative bacteria and notable anaerobic coverage due to its stability against some beta-lactamases. It is commonly used for intra-abdominal infections, gynecological infections, and surgical prophylaxis. Susceptibility testing for cefotetan is important to guide therapy, particularly in light of beta-lactamase-producing pathogens.

Content:

  • Testing Methods:
    • Disk Diffusion (Kirby-Bauer Method):
      • Disks containing cefotetan (30 µg) are placed on Mueller-Hinton agar plates inoculated with the bacterial isolate.
      • After incubation, the diameter of the inhibition zone is measured and interpreted using CLSI or EUCAST guidelines.
    • Minimum Inhibitory Concentration (MIC) Testing:
      • MIC testing is performed using broth microdilution, agar dilution, or automated systems.
      • The MIC is the lowest concentration of cefotetan that inhibits visible bacterial growth.
    • Automated Systems:
      • Platforms like Vitek, Phoenix, or MicroScan may include cefotetan in their panels.

Application:

  • Clinical Use:
    • Cefotetan is used for:
      • Surgical prophylaxis, particularly for colorectal and gynecologic surgeries.
      • Intra-abdominal infections, often combined with metronidazole for enhanced anaerobic coverage.
      • Gynecological infections, including pelvic inflammatory disease (PID).
      • Skin and soft tissue infections caused by susceptible organisms.
      • Urinary tract infections (UTIs) caused by susceptible Enterobacterales.
  • Antimicrobial Stewardship:
    • Encourages the use of cefotetan in targeted therapy, reducing reliance on carbapenems or broader-spectrum cephalosporins.
    • Supports de-escalation of therapy based on susceptibility results.
  • Epidemiological Surveillance:
    • Monitors resistance trends in Enterobacterales and anaerobic pathogens, particularly in beta-lactamase-producing strains.
    • Tracks the emergence of carbapenem-resistant organisms and their impact on cefotetan efficacy.

Cefotetan susceptibility testing is essential for managing infections caused by Gram-negative and anaerobic bacteria, particularly in the context of rising beta-lactamase production. Standardized methods, including disk diffusion and MIC testing, provide reliable results. 

AffiBIOTICS® Cefotetan Ctt 30 µg Disc
CAT# AFG-LFC-0088
Size: 50 Discs
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefotetan Ctt 30 µg Disc
CAT# AFG-LFC-0087
Size: 5x50 Disc
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefotetan Ctt 0.016-256 MIC Test Strip
CAT# AFG-LFC-0558
Size: 10 mic Test
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefotetan Ctt 0.016-256 MIC Test Strip
CAT# AFG-LFC-0557
Size: 100 mic Test
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefotetan Ctt 0.016-256 MIC Test Strip
CAT# AFG-LFC-0556
Size: 30 mic Test
For Price Contact info@affigen.com 0.0 USD