Cefotetan Susceptibility Testing
Cefotetan is a second-generation cephalosporin with extended activity against Gram-negative bacteria and notable anaerobic coverage due to its stability against some beta-lactamases. It is commonly used for intra-abdominal infections, gynecological infections, and surgical prophylaxis. Susceptibility testing for cefotetan is important to guide therapy, particularly in light of beta-lactamase-producing pathogens.
Content:
- Testing Methods:
- Disk Diffusion (Kirby-Bauer Method):
- Disks containing cefotetan (30 µg) are placed on Mueller-Hinton agar plates inoculated with the bacterial isolate.
- After incubation, the diameter of the inhibition zone is measured and interpreted using CLSI or EUCAST guidelines.
- Minimum Inhibitory Concentration (MIC) Testing:
- MIC testing is performed using broth microdilution, agar dilution, or automated systems.
- The MIC is the lowest concentration of cefotetan that inhibits visible bacterial growth.
- Disk Diffusion (Kirby-Bauer Method):
- Automated Systems:
- Platforms like Vitek, Phoenix, or MicroScan may include cefotetan in their panels.
- Automated Systems:
Application:
- Clinical Use:
- Cefotetan is used for:
- Surgical prophylaxis, particularly for colorectal and gynecologic surgeries.
- Intra-abdominal infections, often combined with metronidazole for enhanced anaerobic coverage.
- Gynecological infections, including pelvic inflammatory disease (PID).
- Skin and soft tissue infections caused by susceptible organisms.
- Urinary tract infections (UTIs) caused by susceptible Enterobacterales.
- Cefotetan is used for:
- Antimicrobial Stewardship:
- Encourages the use of cefotetan in targeted therapy, reducing reliance on carbapenems or broader-spectrum cephalosporins.
- Supports de-escalation of therapy based on susceptibility results.
- Epidemiological Surveillance:
- Monitors resistance trends in Enterobacterales and anaerobic pathogens, particularly in beta-lactamase-producing strains.
- Tracks the emergence of carbapenem-resistant organisms and their impact on cefotetan efficacy.
Cefotetan susceptibility testing is essential for managing infections caused by Gram-negative and anaerobic bacteria, particularly in the context of rising beta-lactamase production. Standardized methods, including disk diffusion and MIC testing, provide reliable results.
|
|
|
|
|
|
|
|