Cefiderocol Susceptibility Testing 

Cefiderocol is a novel siderophore cephalosporin antibiotic designed to treat infections caused by multidrug-resistant Gram-negative bacteria, including carbapenem-resistant organisms. Cefiderocol enters bacterial cells by utilizing the siderophore iron transport system, allowing it to overcome resistance mechanisms such as porin loss. It is active against organisms producing extended-spectrum beta-lactamases (ESBLs), AmpC beta-lactamases, and even some metallo-beta-lactamases (MBLs). Susceptibility testing for cefiderocol is critical for ensuring appropriate use and monitoring emerging resistance.

Content:

  • Testing Methods:
    • Broth Microdilution (BMD):
      • CLSI and EUCAST recommend broth microdilution as the gold standard for determining cefiderocol MICs.
      • Iron-depleted cation-adjusted Mueller-Hinton Broth (ID-CAMHB) must be used to simulate physiological conditions for optimal testing.
    • E-test (Gradient Diffusion):
      • Not recommended for cefiderocol due to inconsistent results and lack of standardization.
    • Automated Testing Systems:
      • Automated systems like Vitek or Phoenix may incorporate cefiderocol in their panels; however, validation and calibration for cefiderocol are still ongoing in many systems.

Application:

  • Clinical Use:
    • Cefiderocol is approved for treating:
      • Complicated urinary tract infections (cUTIs).
      • Hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP).
      • Bloodstream infections (BSIs) caused by multidrug-resistant Gram-negative bacteria.
      • Carbapenem-resistant infections due to Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacterales.
  • Antimicrobial Stewardship:
    • Helps guide targeted therapy for multidrug-resistant infections, avoiding unnecessary use of last-line antibiotics.
  • Epidemiological Surveillance:
    • Monitors the emergence of resistance among carbapenemase-producing organisms and other multidrug-resistant pathogens.

Cefiderocol susceptibility testing is essential for managing infections caused by multidrug-resistant Gram-negative pathogens, including carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa.

AffiBIOTICS® Cefiderocol Fdc 30 µg Disc
CAT# AFG-LFC-0070
Size: 50 Discs
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefiderocol Fdc 30 µg Disc
CAT# AFG-LFC-0069
Size: 5x50 Disc
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefiderocol Fdc 0.016-256 MIC Test Strip
CAT# AFG-LFC-0537
Size: 10 mic Test
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefiderocol Fdc 0.016-256 MIC Test Strip
CAT# AFG-LFC-0536
Size: 100 mic Test
For Price Contact info@affigen.com 0.0 USD
AffiBIOTICS® Cefiderocol Fdc 0.016-256 MIC Test Strip
CAT# AFG-LFC-0535
Size: 30 mic Test
For Price Contact info@affigen.com 0.0 USD